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KMID : 0380020200350010072
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Korean Journal of Biotechnology and Bioengineering
2020 Volume.35 No. 1 p.72 ~ p.77
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The Anti-Obesity Effect of Barley Sprout Ethanol Extract
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Park Ye-Seul
Nam Gun-He
Jo Kyung-Jo
Kawk Hye-Won
Kim Myeong-Jin
Kim Jong-Tae
Jang Seung-Hee
Kim Min-Jeong
Kim Young-Min
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Abstract
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Obesity is caused by a variety of reasons, such as diet, genetics, and environmental factors. Here the effect of Barley sprout extract on the differentiation of 3T3-L1 and the expression of adipogenesis was studied. MTT assay for cell toxicity was performed and found to be non-toxic at 3T3-L1, with a survival rate of 90% or higher in Barley sprout extract at all concentrations. The results of oil red O assay, after inducing differentiation by treating the fat formation with insulin, dexamethasone, and IBMX powder-inducing substances at 3T3-L1, showed that the Barley sprout extract inhibited fat formation. The results of a triglyceride assay for the determination of quantitative inhibition of fat formation showed 42.2% and 45.7% in Barley sprout extracts of 50% and 70%, respectively. Western blot was performed to identify the proteins involved in fat cell suppression, and it was found that there was a concentration-dependent decrease in C/EBPα, PPARγ, and p-ACC proteins. Thus, Barley sprout ethanol extract regulates the expression of protein involved in inhibiting fat cells, thereby reducing the differentiation of fat cells and triglyceride production and indicating that it is effective against obesity.
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KEYWORD
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Barley sprout, obesity, adipogenesis, triglyceride
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